Production and optimization of cellulase from Fusarium oxysporum by submerged fermentation
نویسنده
چکیده
Fusarium oxysporum, isolated from infected tomato plant parts, produced maximum cellulase at optimum parameters (pH, 6.0; temp., 50°C; and incubation period, 12 d) in cellulase enzyme production broth having 1% CMC (carboxy methyl cellulose) as a cellulose substrate. Activities of purified cellulases (mol wt, 24, 29 and 45 kDa) were stimulated by concentrations (0-70 mM) of Na and Mg, while EDTA inhibited enzyme activity at all concentrations.
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